Contrasting Nephropathic Responses to Oral Administration of Extract of Cultured Penicillium polonicum in Rat and Primate
نویسندگان
چکیده
Liquid- or solid substrate-cultured Penicillium polonicum administered in feed to rats over several days evokes a histopathological response in kidney involving apoptosis and abnormal mitosis in proximal tubules. The amphoteric toxin is yet only partly characterized, but can be isolated from cultured sporulating biomass in a fraction that is soluble in water and ethanol, and exchangeable on either anion- or cation-exchange resins. After several weeks of treatment renal proximal tubule distortion became striking on account of karyocytomegaly, but even treatment for nearly two years remained asymptomatic. Extract from a batch of solid substrate fermentation of P. polonicum on shredded wheat was incorporated into feed for rats during four consecutive days, and also given as an aqueous solution by oral gavage to a vervet monkey daily for 10 days. Treatment was asymptomatic for both types of animal. Rat response was evident as the typical renal apoptosis and karyomegaly. In contrast there was no such response in the primate; and neither creatinine clearance nor any haematological characteristic or serum component concentration deviated from a control or from historical data for this primate. The contrast is discussed concerning other negative findings for P. polonicum in pigs and hamsters. Renal karyomegaly, as a common rat response to persistent exposure to ochratoxin A, is not known in humans suspected as being exposed to more than the usual trace amounts of dietary ochratoxin A. Therefore the present findings question assumptions that human response to ochratoxin A conforms to that in the rat.
منابع مشابه
Disposition Kinetics of Amoxicillin in Healthy, Hepatopathic and Nephropathic Conditions in Chicken after Single Oral Administration
Fifteen broiler chickens (COBB 400) of 42 days of age weighing 1.8 to 2.0 kg were equally divided into 3 groups, each consisting of 5 birds. Hepatopathy was induced by oral administration of paracetamol while nephropathy was induced by intravenous administration of uranyl nitrate. Kinetic study was investigated in healthy, hepatopathic and nephropathic birds following single oral administration...
متن کاملDetermination of Glycyrrhetic Acid in Rat Plasma by HPLC After Oral Administration of Licorice Aqueous Extract
Licorice is obtained from Glycyrrhiza glabra (G. glabra) in Iran. Glycyrrhizin is the main constituent of G. glabra and has several pharmacological effects. It is hydrolyzed to glycyrrhetic acid (GA) in the intestine after oral administration. In this study, a novel HPLC method was used to determine the concentration of GA in rat plasma after oral administration ...
متن کاملSystematic analysis of main constituents in rat biological samples after oral administration of the methanol extract of Fructus Aurantii by HPLC-ESI-MS/MS
High performance liquid chromatography (HPLC) with diode array detection (DAD) and electrospray ionization tandem mass spectrometry (ESI/MS/MS) was used to analyze the main components in the methanol extract of Fructus Aurantii (FA) and the metabolites in rat biological samples after oral administration of the methanol extract of FA. There were 31 constituents identified in the extract of FA in...
متن کاملSystematic analysis of main constituents in rat biological samples after oral administration of the methanol extract of Fructus Aurantii by HPLC-ESI-MS/MS
High performance liquid chromatography (HPLC) with diode array detection (DAD) and electrospray ionization tandem mass spectrometry (ESI/MS/MS) was used to analyze the main components in the methanol extract of Fructus Aurantii (FA) and the metabolites in rat biological samples after oral administration of the methanol extract of FA. There were 31 constituents identified in the extract of FA in...
متن کاملاثر عصاره گیاه Dendrostellera lessertii بر ترشح TNF-α و تنظیم کاهشی گیرنده های آن بر سطح مونوسیتهای انسانی در محیط کشت
Background : We investigated the mechanism of action of Dendrostellera lessertii effects on decreasing size of adeno-carcinoma of rat intestine and breast tumors. This purpose followed by assessing its effects on TNF-α and TNF-α receptors on cultured human monocytes. Material and method : The breast and intestine tumors were induced in rats by DMBA and DMH, respectively. Human monocytes isolat...
متن کامل